Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

118.1K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
118.1K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

8.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
8.0K
Southern Blot02:57

Southern Blot

24.1K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
24.1K
SDS-PAGE01:27

SDS-PAGE

35.2K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
35.2K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.7K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chitosan or ε-polylysine-functionalized mucoadhesive liposomes for enhanced intracellular delivery of Isoniazid.

International journal of biological macromolecules·2026
Same author

Resolving liquid-to-glass transitions of water under soft nanoconfinement.

Nature communications·2026
Same author

Energy landscape statistics and thermodynamics of a machine-learned model of water.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Restoring the tumour mechanophenotype of vocal fold cancer reverts its malignant properties.

Nature materials·2026
Same author

Collective filament wrapping and nested spiral formation in active polydisperse systems.

Soft matter·2026
Same author

Polymer conformational entropy as the driver of complex coacervation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
Same journal

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.4K

Re-entrant DNA gels.

Francesca Bomboi1, Flavio Romano2, Manuela Leo1,3

  • 1Dipartimento di Fisica, Sapienza Università di Roma, Piazzale A. Moro 2, 00185 Roma, Italy.

Nature Communications
|October 22, 2016
PubMed
Summary
This summary is machine-generated.

Researchers designed a novel DNA hydrogel that self-assembles into complex structures. This biocompatible material exhibits unique phase behavior, melting upon both heating and cooling, offering tunable properties for advanced material development.

More Related Videos

Pouring and Running a Protein Gel by reusing Commercial Cassettes
11:03

Pouring and Running a Protein Gel by reusing Commercial Cassettes

Published on: February 12, 2012

25.4K
Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

794.1K

Related Experiment Videos

Last Updated: Mar 13, 2026

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.4K
Pouring and Running a Protein Gel by reusing Commercial Cassettes
11:03

Pouring and Running a Protein Gel by reusing Commercial Cassettes

Published on: February 12, 2012

25.4K
Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

794.1K

Area of Science:

  • Biomaterials Science
  • Supramolecular Chemistry
  • Nanotechnology

Background:

  • Deoxyribonucleic acid (DNA) is increasingly utilized in material development due to its self-assembly capabilities into intricate supramolecular structures.
  • Translating theoretical models of colloidal particles into experimental DNA nanoconstructs remains an underexplored research area.

Purpose of the Study:

  • To experimentally demonstrate the design of a one-pot DNA hydrogel using competing interactions encoded in DNA sequences.
  • To investigate the unique phase behavior and tunable properties of rationally designed DNA-based bulk materials.

Main Methods:

  • Utilized a carefully selected set of competing interactions encoded within multiple DNA sequences.
  • Fabricated a one-pot DNA hydrogel through self-assembly.
  • Measured relaxation times using light scattering to analyze material dynamics across temperature changes.

Main Results:

  • Successfully designed a DNA hydrogel that exhibits melting behavior upon both heating and cooling.
  • Observed a dramatic slowing of relaxation time within a specific temperature window.
  • Characterized a re-entrant phase diagram, indicative of competing bonding patterns.

Conclusions:

  • It is feasible to rationally design biocompatible bulk materials with unconventional phase diagrams and tunable properties by encoding particle shape and collective response physics into DNA.
  • The study highlights the potential of DNA nanoconstructs for creating advanced materials with predictable and controllable characteristics.