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Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients
Jia Sun1, Yuting Ruan1, Ming Wang2
1Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Scientific Reports
|October 22, 2016
Summary
Circulating long non-coding RNAs (lncRNAs) are altered in obesity, with levels of three specific lncRNAs decreasing. Weight loss interventions increased one lncRNA, suggesting their potential role in obesity.
Area of Science:
- Biochemistry
- Genetics
- Metabolic Diseases
Background:
- Circulating long non-coding RNAs (lncRNAs) are established biomarkers for various human diseases.
- Biomarkers for obesity, particularly lncRNAs, remain largely unidentified.
Purpose of the Study:
- To characterize circulating lncRNA expression profiles in obese versus non-obese individuals.
- To investigate the potential of specific lncRNAs as biomarkers for obesity and their response to weight loss.
Main Methods:
- Genome-wide expression profiling of circulating lncRNAs in blood samples.
- Quantitative RT-PCR validation of candidate lncRNAs.
- Correlation analysis with obesity indicators (BMI, waist circumference, etc.) and insulin resistance.
- Assessment of lncRNA levels following a diet-induced weight loss program.
Main Results:
- Significant downregulation of three lncRNAs (lncRNA-p5549, lncRNA-p21015, lncRNA-p19461) in obese subjects compared to non-obese controls.
- Negative correlation between the levels of these lncRNAs and obesity metrics, including BMI and insulin resistance.
- A significant increase in lncRNA-p19461 levels after a 12-week weight loss intervention.
Conclusions:
- Circulating lncRNAs are deregulated in obesity.
- The observed changes in lncRNA profiles are associated with obesity status and metabolic parameters.
- Weight loss can modulate circulating lncRNA levels, indicating potential mechanistic relevance and therapeutic implications.
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