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Updated: Mar 13, 2026
Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
Published on: June 9, 2022
Histological structure affects recellularization of decellularized arteries.
Jun Negishi1, Yoshihide Hashimoto2, Akitatsu Yamashita3
1Faculty of Textile Science and Technology, Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan; Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0023, Japan; Division of Acellular Tissue and Regenerative Medical Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Decellularized arteries were evaluated for tissue regeneration. Aortic tissue better prevented cell infiltration than radial artery tissue, indicating the elastin lamina
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Decellularized extracellular matrix (ECM) is a promising scaffold for tissue regeneration.
- Understanding the recellularization potential of different vascular tissues is crucial for developing effective grafts.
Purpose of the Study:
- To investigate the in vivo recellularization of decellularized porcine aortas and radial arteries after subcutaneous implantation in rats.
- To compare the cell infiltration and tissue integration capabilities of decellularized aortic and radial artery scaffolds.
Main Methods:
- Porcine aortas and radial arteries were decellularized using high-hydrostatic pressure.
- Cellular removal was confirmed via hematoxylin-eosin staining and residual DNA quantification.
- Decellularized scaffolds were implanted subcutaneously into rats to assess in vivo performance.
Main Results:
- Complete removal of cellular components and preservation of histological structures were achieved in decellularized arteries.
- While cells adhered to all samples, infiltration was primarily observed from the adventitial side in decellularized radial arteries.
- Decellularized aortic tissue demonstrated superior prevention of cell infiltration compared to decellularized radial artery tissue.
Conclusions:
- The elastin lamina in decellularized vascular tissues plays a significant role in preventing cell infiltration.
- Decellularized aortic scaffolds exhibit enhanced properties for controlling recellularization compared to radial artery scaffolds.
- These findings have implications for the design of vascular grafts with controlled regenerative potential.
