Optimization of ultrasonic cell grinder extraction of anthocyanins from blueberry using response surface methodology
Hai-Long Jiang1, Jun-Li Yang2, Yan-Ping Shi2
1Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Abstract:
Ultrasonic cell grinder extraction (UCGE), using water as the solvent, was firstly applied to extract anthocyanins from blueberry. Extraction yield was related with four variables, including ratio of solution to solid, extraction power, buffer time, and extraction time. On the basis of response surface methodology (RSM), the optimal conditions were determined to be the ratio of solution to solid as 25:1(mL/g), the extraction power as 1500W, the buffer time as 3.0s, and the extraction time as 40min. The experimental yield of anthocyanins using UCGE was 2.89mg/g higher than that of conventional ultrasound-assisted extraction (CUAE). This study indicated that UCGE was an innovative, efficient, and environment friendly method in ultrasonic extraction fields, and had a potential to effectively extract other bioactive constituents.
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