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Genetic Transformation of Candida glabrata by Electroporation
Fabian Istel1, Tobias Schwarzmüller1, Michael Tscherner1
1Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
Bio-Protocol
|July 20, 2015
Abstract:
Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata background recipient strain ATCC2001 (Schwarzmüller et al., 2014).
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