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A novel method for serum lipoprotein profiling using high performance capillary isotachophoresis
Estefanía Moreno-Gordaliza1, Sven J van der Lee2, Ayşe Demirkan2
1Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Faculty of Science, Universiteit Leiden, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
Analytica Chimica Acta
|October 26, 2016
Summary
A new capillary isotachophoresis method offers detailed lipoprotein profiling, identifying twice as many species in under 9.5 minutes. This advance aids cardiovascular disease research by enabling better analysis of lipoprotein subtypes.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Separation Science
Background:
- Accurate lipoprotein profiling is crucial for understanding cardiovascular disease.
- Previous methods had limitations in lipoprotein coverage and analysis time.
Purpose of the Study:
- To develop a novel capillary isotachophoresis (cITP) method for enhanced lipoprotein profiling.
- To improve the resolution and speed of lipoprotein analysis in serum or plasma.
Main Methods:
- Development of a novel mixture of 24 spacers and a new neutral-coated capillary.
- Optimization using predictive software and spiked serum samples.
- Validation through comparison with NMR analysis and application to a mouse model.
Main Results:
- The cITP method resolved 18 lipoprotein species in under 9.5 minutes, doubling previous coverage.
- Demonstrated compatibility with lipophilic stains for selective detection.
- Achieved excellent reproducibility with coefficients of variation <2.6%.
Conclusions:
- The new cITP method provides a faster, more comprehensive approach to lipoprotein profiling.
- It enables detailed analysis of lipoprotein particle subtypes, valuable for cardiovascular disease research.
- The method identified diet-induced shifts towards more atherogenic lipoprotein particles in a mouse model.
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