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Protein conformation changes as the result of binding to reversed-phase chromatography column materials
A F Drake1, M A Fung, C F Simpson
1Chemistry Department, Birkbeck College, University of London, U.K.
Journal of Chromatography
|August 4, 1989
Abstract:
The conformational changes of the protein alpha-chymotrypsinogen which may take place on reversed-phase chromatographic material of differing hydrocarbon chain lengths e.g. C4, C6, C8, C10 and phenyl, have been studied by circular dichroism as a function of 1-propanol concentration and pH of the solvent before and after binding to the reversed-phase material.