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Separation and purification of component proteins of the cytochrome P-450-dependent microsomal monooxygenase system
1Institute of Experimental Pathology, German Cancer Research Center, Heidelberg, F.R.G.
Journal of Chromatography
|August 4, 1989
Abstract:
The component proteins of the hepatic microsomal monooxygenase system, including various cytochrome P-450 isozymes, were separated and isolated from liver microsomes of untreated rabbits by Aminohexyl Sepharose and high-performance liquid chromatography (TSK preparative DEAE 5-PW, Bio-Rad HPHT). In addition to the known cytochrome P-450 isozymes, two new isozymes and one variant of the major isozyme were isolated. The monooxygenase activity was reconstituted by incorporating the purified proteins into liposomal membranes.