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08:53
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
Published on: January 12, 2019
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AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes
Yuri Kanno1, Takaya Oikawa2, Yasutaka Chiba1,3
1RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Nature Communications
|October 27, 2016
Summary
Arabidopsis SWEET proteins, AtSWEET13 and AtSWEET14, mediate cellular gibberellin (GA) uptake. A mutant lacking these transporters shows impaired anther dehiscence and altered GA transport, suggesting their role in GA response.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Transmembrane transport of plant hormones is crucial for plant growth and development.
- The precise mechanisms and transporters for gibberellin (GA) transport are not fully understood.
Purpose of the Study:
- To investigate the role of Arabidopsis SWEET proteins in gibberellin (GA) transport.
- To identify potential GA transporters within the SWEET family.
Main Methods:
- Functional expression of AtSWEET13 and AtSWEET14 in yeast and oocytes to assess GA uptake.
- Analysis of a double mutant (sweet13 sweet14) for defects in anther dehiscence and GA transport.
- Phenotypic evaluation of the mutant under exogenous GA treatment and during different developmental stages.
Main Results:
- AtSWEET13 and AtSWEET14 mediate cellular GA uptake when expressed heterologously.
- The sweet13 sweet14 mutant exhibits defective anther dehiscence, which is rescued by exogenous GA.
- Mutant plants show altered long-distance GA transport and modified responses to GA during germination and seedling development.
Conclusions:
- AtSWEET13 and AtSWEET14 function as gibberellin (GA) transporters in Arabidopsis.
- These SWEET proteins play a significant role in modulating GA responses, including anther dehiscence and overall plant development.
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