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GC-MS Analysis of Bio-active Molecules Derived from Paracoccus pantotrophus FMR19 and the Antimicrobial Activity

I Faridha Begum1, R Mohankumar2, M Jeevan3

  • 1Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamilnadu 603 203 India.

Indian Journal of Microbiology
|October 28, 2016
PubMed

Insights

This study identifies eight antimicrobial compounds from Paracoccus pantotrophus FMR19. These compounds show significant antibacterial activity against multidrug-resistant organisms and clinical pathogens.

Area of Science:

  • Microbiology
  • Natural Product Chemistry

Background:

  • Rumen microbiota harbors diverse microorganisms with potential for novel bioactive compound discovery.
  • Antibiotic resistance is a growing global health concern, necessitating the search for new antimicrobial agents.

Purpose of the Study:

  • To investigate the chemical composition of bioactive compounds from Paracoccus pantotrophus FMR19.
  • To evaluate the antibacterial activity of these compounds against multidrug-resistant organisms (MDROs) and clinical pathogens.

Main Methods:

  • Gas Chromatography-Mass Spectrometry (GC-MS) for chemical analysis.
  • Extraction and fractionation of bioactive compounds from bacterial isolate.
  • Antibacterial susceptibility testing against various bacterial strains.

Main Results:

  • GC-MS analysis identified eight major compounds with reported antimicrobial properties from P. pantotrophus FMR19.
  • The hexane fraction contained long chain alkanes, fatty alcohols, fatty acid methyl ester, and aromatic hydrocarbons.
  • The extracted compounds demonstrated efficacy against Salmonella sp., Proteus sp., Metallo β-lactamase, Pan-drug resistant strains, and Methicillin-resistant Staphylococcus aureus (MRSA).

Conclusions:

  • Paracoccus pantotrophus FMR19 is a source of bioactive compounds with broad-spectrum antibacterial activity.
  • These findings suggest potential for developing new therapeutic agents against resistant bacterial infections.
Keywords:
Antibacterial activityBioactive compoundGC–MSMulti-drug resistant organismsParacoccus pantotrophus FMR19Rumen bacteria

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