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Gnotobiotic Human Colon Ex Vivo
Frank D McDermott1, David M A Folan2, Des C Winter3
1UCD School of Veterinary Medicine & Conway Institute of Biomolecular & Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland; UCD School of Medicine and Medical Science, University College Dublin, Belfield, Dublin 4, Ireland; These authors contributed equally to the study.
Gastroenterology Research
|October 28, 2016
Summary
A novel emulsion, ML:8, effectively reduced bacteria in human intestinal tissues. This study demonstrates ML:8
Area of Science:
- Gastroenterology
- Microbiology
- Biotechnology
Background:
- A novel emulsion, ML:8, demonstrated efficacy in eliminating biofilms.
- Commensal bacteria from ex vivo human colonic tissues were utilized.
Purpose of the Study:
- To assess the efficacy and effects of ML:8 against commensal bacteria.
- To evaluate ML:8's impact on human intestinal tissue integrity and function.
Main Methods:
- Human colonic mucosal sheets were treated with saline, povidone iodine, or ML:8.
- Bacterial cultures, electrophysiological measurements, and histological assessments were performed.
Main Results:
- ML:8 virtually abolished viable bacteria, while iodine showed a reduction.
- No significant differences in basal electrophysiology or tissue damage were observed.
- Tissue permeability and carbachol response remained largely intact post-treatment.
Conclusions:
- ML:8 significantly reduced bacterial burden in human intestinal tissues.
- The formulation maintained tissue structural and functional integrity.
- This represents a novel approach for reducing intestinal bacterial load.
