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Spatially multiplexed interferometric microscopy with partially coherent illumination
José Ángel Picazo-Bueno1, Zeev Zalevsky2, Javier García1
1Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
Journal of Biomedical Optics
|October 28, 2016
Summary
Spatially multiplexed interferometric microscopy (SMIM) now uses partially coherent light to enhance quantitative phase imaging. This modification reduces noise and improves phase reconstruction for biological samples.
Area of Science:
- Optical microscopy
- Biomedical imaging
- Holography
Background:
- Spatially multiplexed interferometric microscopy (SMIM) offers a low-cost method to transform standard microscopes into holographic systems.
- The original SMIM method utilized coherent illumination and a diffraction grating for off-axis holographic architecture.
Purpose of the Study:
- To improve quantitative phase imaging in SMIM by implementing partially coherent illumination.
- To reduce coherence noise and enhance phase reconstruction accuracy.
Main Methods:
- Modified SMIM system using partially (temporally reduced) coherent illumination.
- Phase shifting algorithms were employed for phase information recovery instead of off-axis holographic recording.
- A wider field of view limitation (1/2) was implemented compared to the previous scheme (1/3).
Main Results:
- Successfully implemented partially coherent illumination in SMIM.
- Demonstrated improved phase reconstruction due to significant coherence noise reduction.
- Validated the modified technique on diverse biological samples, including cells and microbeads.
Conclusions:
- Partially coherent illumination is a viable modification for SMIM, enhancing quantitative phase imaging capabilities.
- The updated SMIM approach offers improved phase reconstruction and a broader field of view.
- The method is robust and validated across various biological specimens.
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