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Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp
Samiya Khan1, D K Adhikari2, Sanjay Gupta1
1Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., 201301, India.
Objectives:
With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.
Results:
The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.
Conclusions:
Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.
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