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Published on: November 5, 2016
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Decellularized Amniotic Membrane Scaffold as a Pericardial Substitute: An In Vivo Study
J C Francisco1, R Correa Cunha2, M A Cardoso3
1Cell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Curitiba, Paraná, Brazil; Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Curitiba, Paraná, Brazil.
Transplantation Proceedings
|October 30, 2016
Summary
Acellular amniotic membrane (AAM) shows promise as a cost-effective pericardium substitute. This biomaterial integrates well, increasing pericardium thickness and reducing scarring without calcification or significant immune response.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Tissue Engineering
Background:
- Acellular amniotic membrane (AAM) is explored as a novel biomaterial for pericardium substitution.
- AAM offers a cost-effective and abundant matrix for tissue repair applications.
Purpose of the Study:
- To evaluate the efficacy of AAM as a pericardial substitute in a rat model.
- To assess AAM's integration, immunogenicity, and impact on tissue repair.
Main Methods:
- Pericardium excision and substitution with 7-mm AAM patches in Wistar rats (n=10 per group).
- Histopathological analysis (H&E, Alizarin Red S, immunohistochemistry) and scanning electron microscopy after 4 weeks.
Main Results:
- AAM patches maintained structural integrity without observed calcification.
- Thicker pericardium repair tissue formed in the AAM group compared to controls.
- Minimal host-versus-graft reaction due to AAM's low immunogenicity.
Conclusions:
- AAM is a suitable pericardium substitute, integrating well with host tissue.
- AAM reduces adhesions and scarring while increasing pericardium thickness.
- Low immunogenicity and enhanced tissue repair make AAM ideal for pericardial defects.
