Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One Yeast, Sixteen Synthetic Chromosomes, Infinite Possibilities.

Yeast (Chichester, England)·2026
Same author

Synthesis of Programmable Bioelectronic Yeast for Biohybrid Futures.

Engineering biology·2026
Same author

Rolling out <i>Plaque-2-seq</i>: a single plaque sequencing approach enabling rapid, low-cost sequencing of phages directly from plaques.

Microbial genomics·2026
Same author

Environment and Pollen Diversity Differentially Affect the Gut Microbiomes of Introduced Honeybees and Bumblebees.

Evolutionary applications·2026
Same author

Agriculture alters protein evolution of respiratory nitrate reductase in soil bacteria at a global scale.

Environmental research·2026
Same author

Marine heatwaves alter shell microbiomes and denitrification capacity: An oyster family-specific response.

Marine pollution bulletin·2026
Same journal

Mapping the multigenomic human system: structural asymmetry and interface gaps in host-exogenous biological interactions.

Frontiers in microbiology·2026
Same journal

Bacterial resistance across habitats: from German schools to the International Space Station.

Frontiers in microbiology·2026
Same journal

Correction: Unlocking plant growth-promoting traits of endophytic actinobacteria isolated from <i>Anacyclus pyrethrum</i>, an endemic medicinal plant of the Aguelmam azegza region, Morocco.

Frontiers in microbiology·2026
Same journal

Research progress on <i>Avibacterium paragallinarum</i> and related bacterial and viral diseases in poultry and their mixed infections.

Frontiers in microbiology·2026
Same journal

Development and validation of a quantitative method for the enumeration of <i>Salmonella enterica</i> serovar Infantis from environmental poultry feces based on most probable number approach followed by confirmatory qPCR.

Frontiers in microbiology·2026
Same journal

Multi-omics insights into the microbial and metabolic drivers of regional flavor diversity in Guizhou traditional fermented fish.

Frontiers in microbiology·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

11.0K

A Sample-to-Sequence Protocol for Genus Targeted Transcriptomic Profiling: Application to Marine Synechococcus.

Frances D Pitt1, Andrew Millard2, Martin Ostrowski3

  • 1School of Life Sciences, University of Warwick Coventry, UK.

Frontiers in Microbiology
|October 30, 2016
PubMed
Summary
This summary is machine-generated.

This study presents a new ocean sampling protocol for profiling marine bacteria DNA and RNA. Fluorescence-activated cell sorting (FACS) minimizes sample alteration, ensuring reliable data for microbial community analysis.

Keywords:
RNASynechococcusflow cytometryfluorescence activated cell sortingmarine microbiologytranscriptomics

More Related Videos

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

729
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K

Related Experiment Videos

Last Updated: Mar 13, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

11.0K
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

729
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K

Area of Science:

  • Marine microbiology
  • Molecular ecology
  • Genomics and transcriptomics

Background:

  • Whole community metagenomic and metatranscriptomic studies offer insights into marine microbial communities.
  • Standardized protocols are crucial for obtaining reliable environmental survey data.
  • Targeting specific microbial genera requires precise sampling methods.

Purpose of the Study:

  • To develop and validate a comprehensive ocean sample-to-sequence protocol.
  • To enable DNA and RNA profiling of a single bacterial genus using fluorescence-activated cell sorting (FACS).
  • To assess the impact of sampling procedures on transcriptomic profiles.

Main Methods:

  • Development of a complete ocean sample-to-sequence protocol.
  • Utilizing fluorescence-activated cell sorting (FACS) for targeted bacterial genus isolation.
  • Conducting pipeline trials with a cultured isolate, *Synechococcus* sp. BL107.
  • Analyzing the effects of water filtration, sample storage, and cell sorting on RNA profiles.

Main Results:

  • Water filtration showed a limited, well-defined impact on the transcriptomic profile of *Synechococcus* sp. BL107.
  • Sample storage and multiple rounds of cell sorting had negligible effects on RNA sequence profiles.
  • The developed protocol is accessible and reproducible for most researchers.

Conclusions:

  • The described protocol provides a reliable method for targeted DNA and RNA profiling of marine bacteria.
  • FACS-based sampling minimizes experimental artifacts, enhancing data integrity.
  • This approach supports detailed investigations into the functional roles of specific marine microbial taxa.