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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

A Self-Immobilizing and Fluorogenic Probe for β-Lactamase Detection.

Wuyu Mao¹, Lingying Xia¹, Yaqun Wang¹

¹State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P.R. China.

Chemistry, an Asian Journal

|October 30, 2016

Summary

This summary is machine-generated.

Researchers developed a novel fluorogenic probe to detect beta-lactamase activity, an enzyme linked to antibiotic resistance. This probe not only signals enzyme presence but also labels the enzymes, aiding in infectious disease control.

Keywords:

antibiotic resistance enzymes fluorescent probes immobilization β-lactamase

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Area of Science:

Microbiology
Biochemistry
Drug Discovery

Background:

Antibiotic resistance in pathogenic bacteria is a significant global public health threat.
Beta-lactamase enzymes are a primary mechanism bacteria use to resist antibiotic treatments.
Effective monitoring of drug resistance is crucial for controlling infectious diseases.

Purpose of the Study:

To develop a novel self-immobilizing and fluorogenic probe for detecting beta-lactamase activity.
To create a tool for improved monitoring of antibiotic resistance mechanisms.
To facilitate better infectious disease control strategies.

Main Methods:

Development of a self-immobilizing, fluorogenic probe activated by beta-lactamases.
Utilizing the probe's ability to generate a covalent linkage to target enzymes.
Confirmation of covalent enzyme labeling using SDS-PAGE and MALDI-TOF mass spectrometry.
Application of the probe for fluorescent labeling of beta-lactamase-expressing bacteria.

Main Results:

The fluorogenic probe successfully detects beta-lactamase activity by emitting a fluorescence signal.
The probe forms a covalent bond with beta-lactamases and nearby proteins, confirmed by biochemical analyses.
The probe demonstrated utility in fluorescently labeling a variety of bacteria that express beta-lactamases.
A structurally simple probe was developed for enzyme detection and labeling.

Conclusions:

The developed fluorogenic probe is effective for detecting beta-lactamase activity.
This probe offers a dual function of detection and covalent labeling of enzymes.
The probe shows promise as a valuable tool for monitoring antibiotic resistance in bacteria.
This technology can contribute to enhanced infectious disease control efforts.