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In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
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Circadian Rhythm Shapes the Gut Microbiota Affecting Host Radiosensitivity
Ming Cui1, Huiwen Xiao2, Dan Luo3
1Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. cuiming0403@bjmu.edu.cn.
International Journal of Molecular Sciences
|October 30, 2016
Summary
Disrupting the circadian rhythm through abnormal light cycles negatively impacts gut bacteria and reduces radioresistance in mice. This suggests biological clocks are crucial for radiotherapy outcomes.
Area of Science:
- Microbiology
- Chronobiology
- Oncology
Background:
- Modern lifestyles disrupt circadian rhythms, affecting host-microbiota interactions.
- Radiotherapy is a key cancer treatment, but its efficacy concerning circadian rhythm disruption is unclear.
Purpose of the Study:
- To investigate the impact of circadian rhythm disruption on radiotherapy prognosis.
- To analyze changes in intestinal microbiota composition following radiotherapy under varied light/dark cycles.
Main Methods:
- C57BL/6 mice were exposed to different light/dark cycles.
- Intestinal bacterial composition was analyzed using 16S rRNA sequencing.
- Survival rates, body weight, and food intake were measured after irradiation.
Main Results:
- Aberrant light cycles reduced intestinal bacterial diversity and altered gut microbiota composition.
- Mice in disrupted light cycles showed decreased radioresistance compared to controls.
- Changes in enteric bacterial composition varied across different light cycle groups.
Conclusions:
- Circadian rhythm significantly influences gut bacterial composition.
- Disrupted biological clocks impair host radioresistance, impacting radiotherapy prognosis in a preclinical setting.
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