Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

288
After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
288
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

816
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
816
Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

3.7K
Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
3.7K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

6.7K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.7K
Membrane Fluidity01:26

Membrane Fluidity

17.4K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
17.4K
Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)00:53

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

2.3K
Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In Vivo</i> Activity of Antimicrobial Peptoid Oligomers against HSV-1 in a Mouse Model of Herpes Labialis.

ACS infectious diseases·2026
Same author

<i>In-situ</i> structural and electrical conductivity characterization of Sr<sub>2</sub> <i>M</i>MoO<sub>6-δ</sub> double perovskite solid oxide fuel cell anode materials.

ACS applied energy materials·2026
Same author

Morphological and property studies of charged pentablock terpolymer gels containing an aliphatic oil.

Journal of colloid and interface science·2026
Same author

Author Correction: Posttranslational modifications remodel proteome-wide ligandability.

Nature chemical biology·2026
Same author

Posttranslational modifications remodel proteome-wide ligandability.

Nature chemical biology·2026
Same author

Recent advances in mapping protein networks regulated by post-translational modifications via proximity tagging.

Current opinion in chemical biology·2026
Same journal

Metal-Organic Framework Multizyme Colloids with Joint Antioxidant and Protease Function.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Morphology Engineering of Co<sub>3</sub>O<sub>4</sub> via Cetyltrimethylammonium Bromide-Mediated ZIF-67 Synthesis for Efficient Photo-Assisted Electrooxidation of Methanol.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Speciation of Silanol Groups on Commercial Precipitated Silicas via IR Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Hydrogen Plasma-Driven Surface Defect Engineering of ZnO Nanorods: Correlating Electronic Structure and Photoelectrochemical Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Cooperative Self-Assembly of Nanoparticle-Encapsulating Hybrid Protein Cages.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Mar 12, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.9K

Cyclization Improves Membrane Permeation by Antimicrobial Peptoids.

Konstantin Andreev1, Michael W Martynowycz1,2, Andrey Ivankin1

  • 1Department of Physics, Center for Molecular Study of Condensed Soft Matter (μCoSM), Pritzker Institute of Biomedical Science and Engineering, Illinois Institute of Technology , 3440 South Dearborn Street, Chicago, Illinois 60616, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|October 30, 2016
PubMed
Summary
This summary is machine-generated.

Cyclizing antimicrobial peptoids enhances their ability to kill bacteria like Staphylococcus aureus. This structural change improves membrane penetration and therapeutic potential without increasing toxicity.

More Related Videos

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.5K
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
12:02

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Published on: November 2, 2016

12.6K

Related Experiment Videos

Last Updated: Mar 12, 2026

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
10:12

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Published on: September 19, 2022

2.9K
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.5K
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
12:02

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Published on: November 2, 2016

12.6K

Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Materials Science

Background:

  • Natural antimicrobial peptides face limitations in selectivity and bioavailability.
  • Peptidomimetics offer a strategy to improve therapeutic properties.
  • Reducing molecular flexibility can enhance membrane binding affinity.

Purpose of the Study:

  • To investigate the impact of cyclization on antimicrobial peptoid activity and membrane interaction.
  • To compare the efficacy and hemolytic activity of linear versus macrocyclic peptoids.
  • To elucidate the mechanism behind enhanced activity in cyclized peptoids.

Main Methods:

  • Synthesis and characterization of linear and macrocyclic antimicrobial peptoids.
  • In vitro bactericidal assays against Staphylococcus aureus.
  • Hemolytic assays to assess toxicity.
  • Surface X-ray scattering studies on lipid monolayers.

Main Results:

  • Cyclization significantly increased bactericidal activity against Staphylococcus aureus.
  • Macrocyclic peptoids maintained high hemolytic concentrations, indicating low toxicity.
  • Surface X-ray scattering revealed enhanced intercalation of macrocyclic peptoids into anionic lipid monolayers.
  • Cyclization improved membrane penetration efficacy compared to linear analogues.

Conclusions:

  • Macrocyclization is a promising strategy to enhance the therapeutic potential of antimicrobial peptoids.
  • The improved efficacy is attributed to enhanced membrane penetration and binding.
  • Cyclized peptoids demonstrate potential as effective and safe antimicrobial agents.