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Updated: Mar 12, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

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IncFIIk plasmid harbouring an amplification of 16S rRNA methyltransferase-encoding gene rmtH associated with mobile

Racha Beyrouthy1,2,3,4,5, Frederic Robin1,2,3,4,5, Monzer Hamze6

  • 1CHU Clermont-Ferrand, Laboratoire de Bactériologie Clinique, Clermont-Ferrand, France.

The Journal of Antimicrobial Chemotherapy
|November 1, 2016
PubMed
Abstract

Insights

This study reveals a Klebsiella pneumoniae strain with high resistance to antibiotics, featuring a novel plasmid carrying multiple resistance genes, including the first reported plasmid-borne 16S rRNA methyltransferase gene (rmtH).

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Klebsiella pneumoniae is a significant opportunistic pathogen.
  • High-level antibiotic resistance in K. pneumoniae poses a major public health threat.
  • Understanding resistance mechanisms is crucial for effective treatment.

Purpose of the Study:

  • Investigate the resistance mechanisms of Klebsiella pneumoniae strain CMUL78.
  • Characterize the genetic basis for high-level aminoglycoside and β-lactam resistance.
  • Identify novel genetic elements and plasmid structures contributing to antibiotic resistance.

Main Methods:

  • Antibiotic susceptibility testing (disc diffusion, MICs).
  • Molecular characterization of resistance genes (PCR, Sanger sequencing).
  • Plasmid analysis (mating-out assays, PacBio, Illumina sequencing).

Main Results:

  • Strain CMUL78 produces 16S rRNA methyltransferase (RMTase) RmtH, carbapenemase OXA-48, and ESBL SHV-12.
  • A novel IncFIIk plasmid (pRmtH) harbors rmtH and blaSHV-12 genes.
  • A composite transposon (Tn6329) contains a 4-fold tandem repeat of the rmtH-ISCR2 unit.

Conclusions:

  • First report of the 16S RMTase-encoding gene rmtH on a plasmid.
  • Unusual 4-fold tandem repeat organization of rmtH-ISCR2 within a composite transposon.
  • Identified novel plasmid stability modules and a hybrid toxin-antitoxin system.

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