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Lymphocyte proliferative response to rabies virus antigen
G Melino1, C Concato, D Menichella
1Dipartimento di Medicina Sperimentale e Scienze Biochimiche, II Università di Roma Tor Vergata, Italy.
Microbiologica
|July 1, 1989
Summary
Monitoring rabies virus (RV) immunisation requires assessing both antibody (humoral) and lymphocyte (cellular) responses. Cellular response, particularly involving CD4+ T cells, is crucial for effective rabies protection and vaccine monitoring.
Area of Science:
- Immunology
- Virology
- Vaccinology
Background:
- Rabies virus (RV) infection and vaccination induce humoral and cellular immune responses.
- The protective roles of these immune responses remain unclear.
- Effective monitoring parameters for rabies immunisation are needed.
Purpose of the Study:
- To evaluate humoral and cellular immune responses to rabies virus (RV) vaccination.
- To identify valuable monitoring parameters for RV immunisation procedures.
- To assess the correlation between in vivo antibody production and in vitro lymphocyte proliferation.
Main Methods:
- Monitored antibody production, lymphocyte proliferation (CD3, CD4, CD8), and cell surface immunoglobulin expression in response to RV antigen.
- Studied laboratory workers receiving booster vaccine, patients receiving primary vaccination, and healthy volunteers.
- Utilized in vivo and in vitro assays to assess immune responses.
Main Results:
- Booster immunisation and multiple vaccine doses elicited in vitro antigen recognition.
- Proliferation primarily involved CD4+ T cells and B cells.
- Proliferation index correlated well with in vivo antibody production.
- Some individuals, especially those over 65, showed inadequate cellular responses.
Conclusions:
- Cellular immune response should be monitored alongside humoral response for comprehensive rabies vaccine evaluation.
- CD4+ T cell proliferation is a key component of the anti-RV immune response.
- Cellular immunity plays a major role in protection against rabies infection.