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[Determination and analysis of protein profile of different transfer factors]
Guillermo Arturo Guidos-Fogelbach1, Jorge Antonio Paredes-Aguilar, Nayeli Montserrat Colín-Martínez
1Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Laboratorio de Bioquímica Estructural. Ciudad de México, México. guillermoguidos@yahoo.com.
Background:
The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown.
Objectives:
To analyze the protein profiles of different transfer factors marketed in Mexico.
Methods:
6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate.
Results:
The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers.
Conclusion:
Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.
