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Complete Genome Sequences of Three Important Methicillin-Resistant Clinical Isolates of Staphylococcus

Matthew C Riley1, Vincent Perreten2, David A Bemis1

  • 1Department of Biomedical and Diagnostic Sciences, Knoxville, Tennessee, USA.

Genome Announcements
|November 1, 2016
PubMed

Insights

We sequenced three major methicillin-resistant Staphylococcus pseudintermedius clones from North American dogs. These clones, ST68, ST71, and ST84, show distinct genetic elements and antibiotic resistance profiles, aiding in understanding canine infections.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a significant pathogen in canine infections.
  • Understanding the genetic diversity and resistance mechanisms of MRSP is crucial for effective treatment and control.
  • Predominant MRSP clones circulating in North America require detailed genomic characterization.

Purpose of the Study:

  • To perform the first complete genome sequencing of three predominant MRSP clones (ST68, ST71, and ST84) identified in North America.
  • To analyze the genetic differences, including SCCmec elements and antibiotic resistance genes, among these key MRSP clones.
  • To provide a genomic basis for understanding the epidemiology and evolution of MRSP in companion animals.

Main Methods:

  • Whole-genome sequencing of three MRSP isolates representing ST68, ST71, and ST84.
  • Bioinformatic analysis to identify Single Nucleotide Polymorphisms (SNPs), insertion sequences, and mobile genetic elements.
  • Comparative analysis of Staphylococcal Cassette Chromosome mec (SCCmec) types and antibiotic resistance genes (ARGs).

Main Results:

  • Complete genome sequences for MRSP clones ST68, ST71, and ST84 were successfully generated.
  • Distinct SCCmec types were identified in each of the sequenced clones.
  • Variable patterns of antibiotic resistance genes were observed across the three predominant MRSP clones.

Conclusions:

  • The genomic data provide fundamental insights into the genetic makeup of prevalent MRSP clones in North America.
  • Differences in SCCmec elements and ARGs highlight potential mechanisms for MRSP adaptation and transmission in dogs.
  • This study establishes a genomic reference for future research on MRSP pathogenesis and antimicrobial stewardship in veterinary medicine.

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