Related Experiment Video
Updated: May 28, 2026
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Molecular Characterization and Comparative Genomics of Two Staphylococcus pseudintermedius Strains from Humans in
Ola K Elsakhawy1, Haitham Elaadli2, Yassien Badr3
1Department of Veterinary Biomedical Sciences, Rowan University, Glassboro, NJ 08062, USA.
Veterinary Sciences
|May 26, 2026
Summary
Genomic sequencing of Staphylococcus pseudintermedius from human infections in Egypt revealed novel strains. This finding is crucial for understanding zoonotic potential and guiding future public health strategies.
Area of Science:
- Microbiology
- Genomics
- Zoonoses
Background:
- Staphylococcus pseudintermedius, typically found in dogs, is increasingly identified in human infections, raising zoonotic concerns.
- Genomic characterization is vital for understanding antibiotic resistance, pathogenicity, and host adaptation of emerging human pathogens.
Purpose of the Study:
- To perform next-generation genome sequencing and comparative genomic analysis of human S. pseudintermedius isolates from Egypt.
- To assess the genetic relatedness of these isolates to global human S. pseudintermedius populations.
Main Methods:
- Whole-genome sequencing of two human S. pseudintermedius isolates (EGH1, EGH2) using Illumina NovaSeq X Plus.
- Multilocus sequence typing (MLST), pangenome analysis, and antimicrobial resistance gene profiling were conducted.
- Sequence assembly and annotation were performed using Geneious Prime and NCBI Prokaryotic Genome Annotation Pipeline.
Main Results:
- Pangenome analysis revealed a large number of genes, with a significant proportion classified as cloud genes, indicating high genomic diversity.
- MLST identified both isolates as novel sequence types: EGH1 as ST-3037 and EGH2 as ST-2874.
- The study presents the first genomic data for human S. pseudintermedius isolates from Egypt.
Conclusions:
- The genomic data provide insights into the genetic makeup and potential zoonotic capacity of S. pseudintermedius in Egypt.
- Further integrated studies combining genomic, epidemiological, and phenotypic data are necessary to fully understand the implications of these findings.
More Related Videos
07:44Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
Published on: February 14, 2025
08:03A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
Related Concept Videos
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
01:20Applications of Molecular Taxonomy
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
01:25Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
01:28Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
01:29Staphylococcal Skin Infections
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...