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Isolation and Molecular Characterization of Three Staphylococcus pseudintermedius Strains from Dogs and Humans in
Haitham Elaadli1, Yassien Badr2, May Raouf3
1Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 22758, Egypt.
Current Microbiology
|September 6, 2025
Summary
This study characterized Staphylococcus pseudintermedius from dogs and humans in Egypt, revealing diverse antimicrobial resistance and virulence genes. Understanding these genetic traits is crucial for controlling this emerging zoonotic pathogen.
Area of Science:
- Veterinary Microbiology
- Zoonotic Disease Research
- Molecular Epidemiology
Background:
- Staphylococcus pseudintermedius is a significant canine pathogen with increasing recognition as a zoonotic threat.
- Understanding its genetic makeup, including virulence factors and antimicrobial resistance (AMR), is vital for effective control and prevention strategies.
- Limited data exists on the molecular characteristics of S. pseudintermedius isolates from Egypt.
Purpose of the Study:
- To isolate and molecularly characterize Staphylococcus pseudintermedius from canine and human sources in Egypt.
- To identify and compare virulence genes and antimicrobial resistance determinants among the isolates.
- To contribute to the global understanding of S. pseudintermedius as a potential zoonotic agent.
Main Methods:
- Isolation and identification of S. pseudintermedius from clinical samples.
- Molecular characterization using nuc gene sequencing and multilocus sequence typing (MLST).
- Detection of antimicrobial resistance genes (e.g., mecA, blaZ, tetK, tetM) and virulence genes (e.g., coa, sbi/spsK, speta, nanB) via PCR.
Main Results:
- Three S. pseudintermedius isolates (D8 from dog, H10 and H11 from humans) were identified with 100% nuc gene identity to reference strains.
- MLST revealed novel sequence types (STs) for all three isolates.
- Isolates exhibited varied AMR profiles, including beta-lactamase (blaZ) and tetracycline resistance genes (tetK, tetM), alongside resistance to other antibiotic classes.
- All isolates possessed key virulence genes, with isolate H11 uniquely harboring the neuraminidase gene (nanB).
Conclusions:
- Significant genetic diversity in AMR and virulence factors exists among S. pseudintermedius isolates from dogs and humans in Egypt.
- The presence of novel STs and zoonotic potential highlights the need for ongoing global surveillance.
- Molecular characterization is essential for developing targeted interventions against this opportunistic zoonotic pathogen.
