Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

949
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
949
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.1K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

16.1K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
16.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dose-dependent effects of zinc oxide nanoparticles on pre-osteoblast cellular response.

Scientific reports·2026
Same author

Plasticity-Driven Regeneration of Circumvallate Papilla After Lgr5+ Stem Cells Loss.

International journal of biological sciences·2026
Same author

Calcium silicate cement with phosphate-based glass for enhanced osteogenic activity and mechanical strength.

Clinical oral investigations·2026
Same author

Mitochondrial AK3 inhibits nuclear β-catenin localization and its activation through enhancing mitochondrial activity.

Cell death & disease·2026
Same author

Primary cilia in the hypothalamic AgRP neurons mediate metabolic effects of butyrate.

Nature communications·2026
Same author

Fractography of white charcoal reveals past fungal infection and embolism in the secondary xylem via SEM.

Applied microscopy·2026

Related Experiment Video

Updated: Mar 12, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.5K

High-resolution imaging of the microbial cell surface.

Ki Woo Kim1,2

  • 1School of Ecology and Environmental System, Kyungpook National University, Sangju, 37224, Republic of Korea. kiwoo@knu.ac.kr.

Journal of Microbiology (Seoul, Korea)
|November 1, 2016
PubMed
Summary

Microbial cell surfaces, crucial for communication and drug targeting, are visualized using advanced microscopy. Techniques like cryo-field emission SEM and AFM reveal nanoscale structures such (as) hydrophobins and bacteriophages.

Keywords:
imagingmicroscopysurfaceultrastructure

More Related Videos

Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

12.2K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.8K

Related Experiment Videos

Last Updated: Mar 12, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.5K
Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

12.2K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.8K

Area of Science:

  • Microbiology
  • Microbial Cell Biology
  • Nanotechnology

Background:

  • Microorganisms play dual roles as pathogens and decomposers.
  • The microbial cell surface is vital for environmental interaction, communication, and drug targeting.
  • Traditional microscopy methods have limitations in visualizing fine surface structures.

Purpose of the Study:

  • To explore advanced microscopy techniques for visualizing microbial cell surface ultrastructures.
  • To investigate the role of specific surface proteins like hydrophobins.
  • To understand microbial cell-drug interactions and bacteriophage behavior at the nanoscale.

Main Methods:

  • Cryo-field emission scanning electron microscopy (SEM) for high-resolution imaging of cell walls.
  • Atomic force microscopy (AFM) for high-speed live cell imaging in liquid and nanoscale visualization.
  • Transmission electron microscopy (TEM) of freeze-fractured samples to observe surface proteins.

Main Results:

  • Cryo-field emission SEM revealed paired rodlets (hydrophobins) on bacterial and fungal cell walls.
  • AFM enabled clear visualization of cell-drug interactions and bacteriophages.
  • TEM showed hydrophobins with diverse dimensions on fungal spores.

Conclusions:

  • Advanced microscopy techniques offer enhanced resolution and speed for studying microbial cell surfaces.
  • These methods provide new insights into microbial structures, interactions, and responses to external agents.
  • The findings contribute to understanding microbial biology and potential therapeutic targets.