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Intravitreal injection of piperacillin
H C Semple1, J C Liu, G A Peyman
1LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
Ophthalmic Surgery
|August 1, 1989
Summary
Piperacillin is a potential antibiotic for treating bacterial endophthalmitis. Rabbit eye studies show it is safe for intravitreal injection at doses up to 1,500 micrograms, with no observed retinal toxicity.
Area of Science:
- Ophthalmology
- Microbiology
- Pharmacology
Background:
- Bacterial endophthalmitis requires effective intraocular antibiotics.
- Piperacillin is a broad-spectrum antibiotic with established efficacy against Gram-negative and Gram-positive organisms.
Purpose of the Study:
- To assess the safety and toxicity of intravitreal piperacillin in rabbit eyes.
- To determine the maximum tolerated dose of piperacillin for intraocular injection.
Main Methods:
- Piperacillin was administered via intravitreal injection in rabbit eyes at doses ranging from 50 to 3,000 micrograms.
- Histological examination and electroretinograms were used to evaluate intraocular tissue toxicity.
- Toxicity was compared between piperacillin-injected eyes and control eyes injected with balanced saline solution.
Main Results:
- No retinal toxicity was observed with histological examination at doses up to 1,500 micrograms of piperacillin.
- Retinal toxicity in the outer layer was noted at the highest dose of 3,000 micrograms.
- The minimal inhibitory concentration of piperacillin is 800 micrograms/cc for relevant bacterial organisms.
Conclusions:
- Piperacillin demonstrates a favorable safety profile for intravitreal injection in rabbit eyes at doses up to 1,500 micrograms.
- The demonstrated tolerance suggests piperacillin's potential as a safe and effective antibiotic for treating bacterial endophthalmitis in humans.