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Related Concept Videos

Cell Culture01:21

Cell Culture

23.8K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Plant Tissue Culture02:57

Plant Tissue Culture

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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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Related Experiment Video

Updated: Mar 12, 2026

Trypsinizing and Subculturing Mammalian Cells
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Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

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Basic Techniques in Mammalian Cell Tissue Culture.

Katy Phelan1, Kristin M May2

  • 1Cytogenetics Laboratory, Florida Cancer Specialists, Fort Myers, Florida.

Current Protocols in Toxicology
|November 2, 2016
PubMed
Summary
This summary is machine-generated.

Learn essential mammalian cell culture skills for preserving cell structure and function. This guide covers aseptic techniques, media preparation, passaging, freezing, and viability assessment for reliable cell biology research.

Keywords:
aseptic techniquefreezing cellsmammalian cellsmediumpassaging cellstissue culture

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
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Area of Science:

  • Cell Biology
  • Biotechnology

Background:

  • Mammalian cell cultures are fundamental tools in biological research.
  • Maintaining cellular integrity, function, and behavior in vitro requires specialized techniques.

Purpose of the Study:

  • To outline the core skills necessary for effective mammalian cell culture.
  • To provide a practical guide for researchers on preserving cell viability and characteristics.

Main Methods:

  • Aseptic technique maintenance
  • Customized media preparation
  • Cell passaging protocols
  • Cryopreservation and thawing procedures
  • Viable cell counting methodologies

Main Results:

  • Successful preservation of cell structure, function, and behavior in culture.
  • Establishment of reliable protocols for cell culture maintenance and storage.
  • Ensured viability and integrity of cultured cells through proper handling.

Conclusions:

  • Proficiency in basic cell culture techniques is crucial for reproducible research outcomes.
  • Standardized methods for cell culture, including freezing and recovery, are vital for long-term studies.
  • This unit provides a foundational skill set for researchers working with cultured mammalian cells.