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Published on: November 5, 2013
Fluorescent Nanocomposite for Visualizing Cross-Talk between MicroRNA-21 and Hydrogen Peroxide in
Limin Yang1, Yanfei Ren1, Wei Pan1
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University , Jinan 250014, People's Republic of China.
Abstract:
MicroRNAs (miRNAs) and reactive oxygen species (ROS) are concurrently implicated in heart ischemia-reperfusion (IR) injury. There may exist mutual cross-talk between miRNAs and ROS in cardiac IR injury process. In this study, we developed a novel crown-like silica@polydopamine-DNA-CeO2 nanocomposite by assembly of silica@polydopamine-DNA1 nanoparticles decorated with satellite CeO2-DNA2 nanoparticles for detecting and imaging of microRNA-21 (miR-21) and hydrogen peroxide (H2O2) in simulated IR injury in living cells and in vivo. The miRNA-21 was found to be regulated by H2O2 via PI3K/AKT signaling pathway for the first time in H9C2 cells in simulated ischemia-reperfusion injury. H2O2 and miRNA-21 are overproduced during mimicked heart ischemia-reperfusion injury, suggesting that they are closely related to reperfusion injury. All these results reveal that there is definite cross-talk between miR-21 and H2O2 in IR injury. The current method can provide a promising strategy to further explore the interplaying roles between ROS and miRNAs in other pathological processes.
