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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

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Related Experiment Video

Updated: Mar 12, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

microRNA Target Prediction.

William Ritchie¹

¹CNRS, UMR 5203, Montpellier, 34094, France. W.Ritchie@centenary.org.au.

Methods in Molecular Biology (Clifton, N.J.)

|November 4, 2016

Summary

This summary is machine-generated.

MicroRNAs regulate gene expression by targeting genes. New methods refine computational predictions of microRNA targets by integrating expression data, improving accuracy for identifying true targets.

Keywords:

Target genes microRNA microRNA expression

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Last Updated: Mar 12, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

microRNAs (miRNAs) are key regulators of gene expression.
Computational tools predict miRNA targets but often yield excessive results.
Accurate identification of functional miRNA targets is crucial for understanding gene regulation.

Purpose of the Study:

To develop and describe procedures for refining miRNA target predictions.
To enhance the accuracy of identifying true miRNA targets over putative ones.
To integrate computational predictions with experimental expression data.

Main Methods:

Utilizing commonly used miRNA prediction software.
Integrating miRNA prediction results with publicly available gene expression data.
Developing refined procedures for target identification.

Main Results:

The described procedures effectively refine large sets of predicted miRNA targets.
Integration of expression data significantly improves the specificity of target prediction.
The refined methods prioritize the identification of genuine miRNA-gene interactions.

Conclusions:

The developed procedures offer a more accurate approach to identifying functional miRNA targets.
These methods are particularly valuable for research prioritizing true target identification.
This work advances the understanding of miRNA-mediated gene regulation.