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Validation of Biomarker Proteins Using Reverse Capture Protein Microarrays
Catherine Jozwik1, Ofer Eidelman1, Joshua Starr1
1Department of Anatomy, Physiology and Genetics, Institute for Molecular Medicine, Center for Medical Proteomics, Uniformed Services University School of Medicine, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 4, 2016
Summary
Reverse capture protein microarrays excel at identifying low-abundance disease biomarkers in biological fluids. This technology enables quantitative assessment for diagnostic and prognostic validation of antigens.
Area of Science:
- Proteomics
- Biomarker Discovery
- Molecular Diagnostics
Background:
- Genomics and high-throughput sequencing have identified numerous proteins.
- Protein chip technology offers miniaturized, parallel screening for protein analysis.
- Reverse capture protein microarrays are ideal for detecting low-abundance biomarkers in complex biological samples.
Purpose of the Study:
- To describe methods for applying reverse capture protein microarray technology.
- To highlight contributions to the development and application of this microarray platform.
- To focus on the use of body fluids beyond serum/plasma for biomarker discovery.
Main Methods:
- Utilizing reverse capture protein microarrays for high-throughput screening.
- Probing slides with specific antibodies to detect target biomarkers.
- Quantitatively determining antibody titers for diagnostic and prognostic validation.
- Analyzing various biological fluids including blood, saliva, urine, and cerebrospinal fluid.
Main Results:
- Demonstrated the effectiveness of reverse capture protein microarrays in identifying disease-specific biomarkers.
- Enabled quantitative assessment of biomarker utility through antibody titer determination.
- Facilitated the statistical validation of antigens as diagnostic or prognostic markers.
- Showcased successful applications in body fluids other than serum/plasma.
Conclusions:
- Reverse capture protein microarrays are a powerful platform for biomarker discovery and validation.
- The technology is crucial for identifying low-abundance proteins in complex biological matrices.
- Future advancements will enhance its role in discovery science for signaling proteins.
