Robust Ki67 detection in human blood by flow cytometry for clinical studies

Area of Science:

• Immunology
• Biomarker Discovery
• Flow Cytometry

Background:

• Ki67 protein is a crucial prognostic and predictive biomarker in various cancers.
• Flow cytometry offers advanced capabilities for single-cell, multiparameter analysis.

Purpose of the Study:

• To develop and validate a multicolor quantitative flow cytometry assay for intracellular Ki67 detection.
• To assess the assay's precision and the stability of Ki67 expression in collected blood samples.

Main Methods:

• Development of a multicolor quantitative flow cytometry assay.
• Optimization and validation of the assay for intracellular Ki67 detection in human blood immune cell subsets.
• Evaluation of sample stability up to 72 hours post-collection using Cyto-Chex® BCT tubes.

Main Results:

• The developed flow cytometry assay demonstrated excellent precision for intracellular Ki67 detection.
• Sample stability assessments showed minimal percentage changes (within 20%) for key results up to 72 hours post-collection.
• The assay is robust for analyzing clinical samples.

Conclusions:

• The validated Ki67 flow cytometry assay is suitable for clinical sample analysis.
• Peripheral blood accessibility allows for continuous Ki67 monitoring.
• This assay can serve as a valuable biomarker in immunotherapy studies and other clinical research.