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Peptide-Stabilized, Fluorescent Silver Nanoclusters: Solid-Phase Synthesis and Screening.

Simon Gregersen1, Tom Vosch2, Knud J Jensen1

  • 1Department of Chemistry, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 4, 2016
PubMed
Summary

Solid-phase synthesis enables rapid screening of peptide ligands for fluorescent silver nanoclusters (AgNCs). This method identifies novel peptide-stabilized AgNCs (P-AgNCs) with tunable properties for bioimaging applications.

Keywords:
fluorescenceon-resin screeningpeptidessilver nanoclusterssolid-phase synthesis

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Area of Science:

  • * Nanomaterials Science
  • * Peptide Chemistry
  • * Solid-Phase Synthesis

Background:

  • * Few-atom silver nanoclusters (AgNCs) display strong fluorescence but require stabilizing ligands.
  • * Peptide ligands are less explored than DNA for AgNC stabilization.
  • * Solution-phase synthesis limits throughput for ligand screening.

Purpose of the Study:

  • * To develop a high-throughput solid-phase method for synthesizing and evaluating peptide-stabilized AgNCs (P-AgNCs).
  • * To screen a peptide library for novel fluorescent P-AgNCs.
  • * To investigate the impact of peptide characteristics on AgNC stability and fluorescence.

Main Methods:

  • * Employed 9-fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis on hydroxymethyl-benzoic acid (HMBA) functionalized PEGA resin.
  • * Synthesized and screened a 144-member peptide library for P-AgNC formation and properties.
  • * Evaluated fluorescence intensity and chemical stability of P-AgNCs.

Main Results:

  • * Identified novel peptide ligands yielding fluorescent AgNCs.
  • * Demonstrated the feasibility of on-resin screening and evaluation of P-AgNCs.
  • * Assessed the influence of peptide length, charge, and cysteine content on AgNC stabilization.

Conclusions:

  • * Solid-phase synthesis significantly enhances throughput for P-AgNC development.
  • * This approach facilitates the discovery of new fluorescent P-AgNCs.
  • * Findings support future spectroscopic studies for bioimaging applications.