Using [U-¹³C⁶]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts

Metabolic flux analysis (MFA) is a comprehensive technique that allows researchers to create a map of cellular metabolic state. This method is extensively studied in the literature in the context of the metabolism of various cancer cells, and it normally utilizes a labeled substrate that is absorbed by the cells, the levels of the incorporation are measured by mass spectrometry (MS) within the pool of metabolites and computational estimation is performed. Here, we propose the use of this assay to study metabolic changes that occur in oncogene-induced senescence (OIS) of normal human fibroblasts (Wi38) versus those in the state of proliferation/quiescence.