Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesh Fixation in Ventral Rectopexy: Is Rectal Anchoring Really Justified?

Diseases of the colon and rectum·2026
Same author

[Acute appendicitis : can surgery be avoided ?]

Revue medicale suisse·2026
Same author

[Immersion in a surgical training program in Burundi for posterior perineal reconstruction].

Revue medicale suisse·2026
Same author

ESGO Statements on Opportunistic Salpingectomy for Prevention of Tubo-Ovarian Carcinoma.

JAMA·2026
Same author

Prophylactic three-dimensional funnel mesh for prevention of parastomal hernia: propensity-score-matched cohort study.

BJS open·2026
Same author

Mesh rectopexy in rectal prolapse: toward consensus or continued controversy?

Techniques in coloproctology·2026
Same journal

Early-Life Cardiovascular Programming by Maternal Protein Restriction: Electrophysiological, Structural, and Molecular Alterations in Female Rats.

Experimental cell research·2026
Same journal

The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

Experimental cell research·2026
Same journal

Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

Experimental cell research·2026
Same journal

Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

Experimental cell research·2026
Same journal

MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

Experimental cell research·2026
Same journal

Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

Experimental cell research·2026
See all related articles

Related Experiment Video

Updated: Mar 12, 2026

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
08:22

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Published on: December 16, 2021

8.3K

An optimized method for mouse liver sinusoidal endothelial cell isolation.

Jeremy Meyer1, Stéphanie Lacotte2, Philippe Morel1

  • 1Division of Digestive and Transplantation Surgery, University Hospitals of Geneva, Rue Gabrielle-Perret-Gentil 4, 1211 Genève 14, Switzerland; Unit of Surgical Research, University of Geneva, Rue Michel-Servet 1, 1206 Genève, Switzerland.

Experimental Cell Research
|November 6, 2016
PubMed
Summary
This summary is machine-generated.

This study presents a new, reliable method for isolating high-purity mouse liver sinusoidal endothelial cells (LSEC), yielding more cells with less contamination for biological research.

Keywords:
CD11bLSECMicePurificationStabilin-2

More Related Videos

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
08:54

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Published on: February 12, 2018

52.5K
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
12:35

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

Published on: November 10, 2011

19.8K

Related Experiment Videos

Last Updated: Mar 12, 2026

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
08:22

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Published on: December 16, 2021

8.3K
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
08:54

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Published on: February 12, 2018

52.5K
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
12:35

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

Published on: November 10, 2011

19.8K

Area of Science:

  • Cell biology
  • Immunology
  • Hepatology

Background:

  • Liver sinusoidal endothelial cells (LSEC) play crucial roles in liver function and immunity.
  • Accurate isolation of pure LSEC is essential for studying their biological functions.
  • Existing LSEC isolation methods have limitations in yield and purity.

Purpose of the Study:

  • To develop and validate an improved method for isolating pure mouse LSEC.
  • To compare the efficiency of the new method against established techniques.
  • To provide a reliable protocol for obtaining high-quality LSEC for research.

Main Methods:

  • Modified two-step collagenase digestion and Optiprep density gradient centrifugation.
  • Comparison of LSEC purification using CD11b depletion with long-term adherence versus CD146+ MACS and short-term adherence.
  • Scanning electron microscopy to verify LSEC morphology and fenestrations.

Main Results:

  • The developed technique yielded significantly higher numbers of LSEC (7.07±3.80 million/liver) compared to CD146+ MACS (2.94±1.28 million) and short-term adherence (0.99±0.66 million).
  • Achieved high purity of LSEC (95.10±2.58%) with minimal macrophage contamination (1.87±0.77%).
  • Isolated LSEC exhibited characteristic fenestrations, confirming their identity and integrity.

Conclusions:

  • A reliable and reproducible protocol for isolating high-yield, high-purity mouse LSEC has been established.
  • This method surpasses existing techniques in terms of cell yield and purity.
  • The protocol facilitates the study of LSEC biological functions through efficient cell preparation.