Novel MWCNTs/graphene oxide/pyrogallol composite with enhanced sensitivity for biosensing applications

Area of Science:

• Electrochemistry
• Materials Science
• Analytical Chemistry

Background:

• Omeprazole (OME) determination is crucial for pharmaceutical quality control and clinical analysis.
• Developing sensitive and selective electrochemical sensors is essential for accurate OME quantification.
• Graphene oxide (GO), multiwalled carbon nanotubes (MWCNTs), and pyrogallol (PG) offer promising properties for electrochemical sensing applications.

Purpose of the Study:

• To fabricate and characterize a novel electrochemical biosensor for the sensitive determination of omeprazole (OME).
• To investigate the synergistic effects of GO, MWCNTs, and PG in enhancing OME detection.
• To evaluate the sensor's performance in real-world samples, including pharmaceutical formulations and human serum.

Main Methods:

• Fabrication of a modified carbon paste electrode (CPE) using graphene oxide nano-sheets (GO), multiwalled carbon nanotubes (MWCNTs), and pyrogallol (PG).
• Characterization of the composite material's morphology and structure using various techniques.
• Optimization of sensor composition and measurement conditions using experimental design.
• Electrochemical analysis of omeprazole using differential pulse voltammetry (DPVs).

Main Results:

• The modified electrode exhibited significantly enhanced anodic oxidation activity for OME compared to the bare CPE.
• DPVs revealed two linear dynamic ranges for OME detection: 2.0×10^-10-6.0×10^-6M and 6.0×10^-6-1.0×10^-4M.
• A highly sensitive detection limit of 1.02×10^-11M was achieved at pH 7.
• Accurate determination of OME in pharmaceutical formulations (98.58% recovery) and human serum (100.97% recovery) was demonstrated.

Conclusions:

• The developed GO-MWCNT-PG modified electrode is a highly sensitive and reliable platform for omeprazole determination.
• The sensor shows excellent potential for clinical analysis, quality control, and routine drug monitoring.
• The synergistic integration of nanomaterials and pyrogallol significantly boosts electrochemical sensing performance.