Related Experiment Video

Updated: Mar 12, 2026

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
10:50

Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding

Published on: September 15, 2010

10.0K

Dynamic conformational changes in the rhesus TRIM5α dimer dictate the potency of HIV-1 restriction

Rajan Lamichhane1, Santanu Mukherjee2, Nikolai Smolin3

  • 1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.

Virology
|November 9, 2016
PubMed

Insights

Rhesus TRIM-alpha5 (rhTRIM5α) protein restricts HIV-1 by destabilizing the viral core. Dynamic conformational changes in its L2 region are crucial for this antiviral restriction mechanism.

Area of Science:

  • Virology
  • Structural Biology
  • Immunology

Background:

  • The TRIM5α protein from rhesus macaques (rhTRIM5α) inhibits HIV-1 infection.
  • This inhibition involves the premature disassembly of the viral core.
  • Alpha-helical elements in the Linker 2 (L2) region of TRIM5α affect its restriction potency.

Purpose of the Study:

  • To investigate the dynamic conformational changes in the L2 region of rhTRIM5α.
  • To understand the role of these conformational changes in HIV-1 restriction.

Main Methods:

  • Single-molecule Förster Resonance Energy Transfer (smFRET) analysis.
  • Analysis of restriction-modulating mutations in the L2 region.

Main Results:

  • The L2 region of rhTRIM5α dimers undergoes dynamic conformational changes, displacing L2 regions by 25 angstroms.
  • Mutations that impair restriction prevent these dynamic changes and stable alpha-helical conformations in the L2 region.

Conclusions:

  • Conformational changes in the L2 region are essential for rhTRIM5α's antiviral activity.
  • These changes likely mediate the displacement of SPRY domains, leading to capsid destabilization and HIV-1 restriction.
Keywords:
Coiled coilDimerHIV-1Molecular dynamics simulationRestriction factorSingle molecule FRETTRIM5alphaTripartite MotifsmFRET

More Related Videos

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

14.8K
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

16.3K

Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.5K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
978