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Related Concept Videos

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Related Experiment Video

Updated: Mar 12, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs).

Stuart Cantsilieris1, Holly A Stessman2, Jay Shendure2,3

  • 1Department of Genome Sciences, University of Washington School of Medicine, 3720 15th Ave NE, S413C, Box 355065, Seattle, WA, 98195-5065, USA. cantss@u.washington.edu.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2016
PubMed
Summary
This summary is machine-generated.

Molecular inversion probes (MIPs) offer an economical method for targeted DNA sequencing. This study details a protocol for capturing over 2000 genomic targets from 192 samples using MIPs and high-throughput sequencing.

Keywords:
Exonuclease cleanup and gel electrophoresisMassively parallel sequencingMolecular inversion probesReal-time PCR

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Targeted DNA sequencing is crucial for genomic research.
  • Existing methods can be costly and complex.
  • Molecular inversion probes (MIPs) offer a promising alternative.

Purpose of the Study:

  • To present a detailed "wet bench" protocol for targeted genomic DNA capture and sequencing.
  • To demonstrate the scalability and cost-effectiveness of MIPs for large-scale studies.

Main Methods:

  • Utilized molecular inversion probes (MIPs) with unique targeting arms and universal linkers for selective DNA capture.
  • Incorporated sequencing adaptors and sample-specific barcodes for large-scale pooling.
  • Performed high-throughput sequencing on an Illumina HiSeq 2000 platform.

Main Results:

  • Successfully captured and sequenced over 2000 genomic targets.
  • Processed 192 samples efficiently, representative of a single sequencing lane.
  • Demonstrated a cost-effective approach for targeted sequencing.

Conclusions:

  • The described MIP-based protocol is a versatile and economical tool for targeted genomic sequencing.
  • This method enables high-throughput analysis of numerous samples and targets.
  • The protocol is suitable for various genomic applications requiring cost-effective targeted sequencing.