Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 12, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Methods for Genotyping-by-Sequencing.

Beth A Rowan1, Danelle K Seymour1, Eunyoung Chae1

  • 1Department of Molecular Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 37-39, 72076, Tübingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2016
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biosynthetic gene clusters in <i>Pseudomonas viridiflava</i> have a fitness cost during <i>Arabidopsis thaliana</i> infection.

mSystems·2026
Same author

Seasonality, land use and plant community diversity shape microbiome-pathogen interactions in wild populations of Arabidopsis thaliana.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same author

PLANeT: Understanding and leveraging the genome of land plants for a sustainable future.

Cell·2026
Same author

Specialization of independently acquired flagellar FliC proteins in plant-associated <i>Sphingomonas</i> balances swimming and immunogenicity.

bioRxiv : the preprint server for biology·2026
Same author

Specialization of independently acquired flagellar FliC proteins in plant-associated <i>Sphingomonas</i> balances swimming and immunogenicity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Rapid adaptation and extinction in synchronized outdoor evolution experiments of <i>Arabidopsis</i>.

Science (New York, N.Y.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Modern sequencing technologies, like genotyping-by-sequencing (GBS), accelerate genetic mapping. We present two Illumina-based GBS protocols to efficiently discover genes linked to traits in diverse species.

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Understanding the gene-phenotype connection is crucial in biology.
  • Genetic mapping is a key method for identifying genes responsible for specific traits.
  • Traditional genotyping methods were a bottleneck in genetic mapping studies.

Purpose of the Study:

  • To present two streamlined genotyping-by-sequencing (GBS) protocols for genetic mapping.
  • To enable efficient and cost-effective genetic analysis across various species.
  • To facilitate large-scale population genetic studies.

Main Methods:

  • Developed two GBS protocols utilizing the Illumina sequencing platform.
  • Protocol 1: Genome-wide genotyping of selected marker positions via restriction digestion.
Keywords:
GBSGenetic mappingGenomic DNARAD-seqRESCANReduced-representationSequencing librarySolid phase reverse immobilization

More Related Videos

Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.2K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

40.0K

Related Experiment Videos

Last Updated: Mar 12, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K
Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.2K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

40.0K
  • Protocol 2: Preparation of cost-effective paired-end whole-genome sequencing libraries.

    • Main Results:

    • The presented protocols significantly reduce time, labor, and cost associated with genetic mapping.
    • GBS enables high-resolution genetic mapping and surveys genetic variation in natural populations.
    • Protocols are adaptable for different species and can be integrated with liquid-handling robotics.

    Conclusions:

    • The developed GBS protocols offer a powerful and accessible approach for genetic mapping and population genetics.
    • These methods advance the ability to link genes to phenotypes efficiently.
    • The protocols are suitable for a broad range of genotyping applications in diverse organisms.