Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evidence for the prognostic value of TP53 mutations in circulating tumor DNA across solid malignancies: a systematic review and meta-analysis.

BMC cancer·2026

Same author

Enhanced detection of bladder cancer using combined circulating tumor cells, urine-derived epithelial cells, and molecular biomarkers.

Journal of cancer research and clinical oncology·2026

Same author

A multisource cue fusion-based method for coronary artery stenosis detection in digital subtraction angiography.

Journal of thoracic disease·2026

Same author

Infiltrative hepatocellular carcinoma Resistance: Integrating Microenvironment, Host Factors, and Therapy.

Clinical and molecular hepatology·2026

Same author

TRIM47-facilitated PLK1 stabilization promotes the proliferation of liver cancer cells.

Cellular oncology (Dordrecht, Netherlands)·2025

Same author

Erratum: [Corrigendum] Evaluation of the inhibitory effects of vaginal microorganisms on sperm motility <i>in vitro</i>.

Experimental and therapeutic medicine·2025

Same journal

Epigenetic plasticity and chemoresistance in cancer: mechanisms, biomarkers, and translational opportunities for real-world evidence.

Frontiers in cell and developmental biology·2026

Same journal

Matched embryo-endometrium RNA-seq reveals coordinated but asymmetric transcriptomic reprogramming at the onset of early equine pregnancy.

Frontiers in cell and developmental biology·2026

Same journal

Myelin development in the peripheral nervous system of <i>Trachemys scripta</i>.

Frontiers in cell and developmental biology·2026

Same journal

A deep learning-based classification method for subclinical zonular laxity in AS-OCT images.

Frontiers in cell and developmental biology·2026

Same journal

Advancing fat graft survival: from adipose-derived stem cell mechanisms to next-generation regenerative strategies.

Frontiers in cell and developmental biology·2026

Same journal

CRISPR-based next-generation molecular diagnostics for bone infection.

Frontiers in cell and developmental biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 12, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Single Cell Isolation and Analysis.

Ping Hu¹, Wenhua Zhang², Hongbo Xin¹

¹The Center for Biotechnology and Biopharmaceutics, Institute of Translational Medicine, Nanchang University Nanchang, China.

Frontiers in Cell and Developmental Biology

|November 10, 2016

Summary

This summary is machine-generated.

Understanding individual cell differences is crucial for targeted therapies. Single cell analysis provides detailed insights for precision medicine, moving beyond population averages.

Keywords:

analysis heterogeneity isolation sequencing single cell

More Related Videos

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

Related Experiment Videos

Last Updated: Mar 12, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

Published on: November 4, 2021

Area of Science:

Biotechnology
Genomics
Cell Biology

Background:

Individual cell heterogeneity significantly impacts population function and fate.
Conventional assays analyze average responses, masking critical cell-to-cell variations.
Understanding these differences is vital for advancements in cancer, immunology, and developmental biology.

Approach:

This review focuses on recent advancements in single cell isolation and analysis technologies.
It covers key technologies, analytical methods, and their primary applications.
The review also summarizes the historical context and limitations of these techniques.

Key Points:

Single cell analysis reveals variations in RNA transcripts and protein expression.
These variations are essential for understanding cellular responses to drugs and treatments.
This approach enables more informed therapeutic decision-making in precision medicine.

Conclusions:

Single cell isolation and analysis technologies are rapidly evolving.
These technologies offer unprecedented insights into cellular heterogeneity.
Further development holds significant potential for personalized medicine and biological research.