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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Performing Custom MicroRNA Microarray Experiments
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MicroRNA Expression Profiling Using Agilent One-Color Microarray.

Carmela Dell'Aversana1,2, Cristina Giorgio3, Lucia Altucci4,3

  • 1Institute of Genetics and Biophysics, CNR, 80131, Naples, Italy. dellaversana@igb.cnr.it.

Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2016
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) expression profiling identifies regulatory changes in health and disease. This study details microarray methods for accurate miRNA expression analysis using Agilent SurePrint technology.

Keywords:
Expression profileMicroarrayPlatformSlidemiRNA

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Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • MicroRNA (miRNA) expression profiling is crucial for understanding physiological and pathological processes.
  • miRNA analysis has significant applications in diagnostics for cancer, cardiovascular diseases, autoimmune disorders, and forensics.

Purpose of the Study:

  • To describe the workflow and methods for miRNA expression profiling.
  • To focus on microarray-based profiling using Agilent SurePrint technology and One-Color Microarray analysis.

Main Methods:

  • Utilizing Agilent SurePrint microarray technology for high-throughput genomic approaches.
  • Implementing the One-Color Microarray method for miRNA expression profiling.

Main Results:

  • Detailed description of the workflow for miRNA expression profiling.
  • Explanation of the methods for expression profiling using Agilent One-Color Microarray.

Conclusions:

  • Microarray-based miRNA expression profiling provides a robust method for molecular diagnostics.
  • Agilent SurePrint technology offers an effective platform for comprehensive miRNA analysis.