Related Experiment Video
Updated: Mar 12, 2026
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes
Il Joon Lee1, Nam-In Goo1, Dong-Eun Kim1
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. kimde@konkuk.ac.kr.
Abstract:
We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes. This system is a label/quencher-free fluorescence enhancement system based on rolling circle amplification (RCA)-responsive G-quadruplex formation, which enables a highly selective detection of T790M SNPs in the gene encoding the epidermal growth factor receptor, EGFR.
