Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial assembly of collagen and poly(acrylic acid) forms a dense and low-permeable collagen membrane.

Biochemical and biophysical research communications·2026
Same author

Localized stiffness programming enables tunable spatial control of vascular density in 3D hydrogels.

Chemical communications (Cambridge, England)·2026
Same author

Bubble Formation Control: Fabrication of Centimeter-Sized Tissue-Like Constructs by Catalase-Coated Oxygen-Releasing Hydrogel.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Organic acid optimization for the fabrication of centimeter-scale uniaxially aligned capillary networks in cell-laden collagen fiber assemblies.

Biochemical and biophysical research communications·2026
Same author

Phototunable hydrogel mechanics for spatial guidance of blood capillary morphogenesis.

Biofabrication·2026
Same author

Neonicotinoid pesticides disrupt gingival epithelial barrier function.

Toxicology reports·2026

Related Experiment Video

Updated: Mar 12, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K

Development of Microfluidic Systems for Fabricating Cellular Multilayers.

Koji Matsuura1, Ikuyo Sugimoto, Yuka Kuroda

  • 1Research Core for Interdisciplinary Sciences, Okayama University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|November 11, 2016
PubMed
Summary

A novel microfluidic system simplifies cellular multilayer fabrication, reducing labor and cell stress. This innovation enhances extracellular matrix coating for tissue modeling and pharmaceutical applications.

More Related Videos

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices
06:21

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices

Published on: January 25, 2021

3.4K
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
09:56

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Published on: September 29, 2015

9.9K

Related Experiment Videos

Last Updated: Mar 12, 2026

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
10:18

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Published on: January 27, 2017

15.2K
Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices
06:21

Design and Development of a Three-Dimensionally Printed Microscope Mask Alignment Adapter for the Fabrication of Multilayer Microfluidic Devices

Published on: January 25, 2021

3.4K
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
09:56

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Published on: September 29, 2015

9.9K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Traditional methods for coating cells with extracellular matrices (e.g., centrifugation) are labor-intensive and can apply significant mechanical force to cells.
  • Developing efficient and gentle methods for fabricating cellular multilayers is crucial for advancing tissue engineering and cell-based assays.

Purpose of the Study:

  • To design and validate a microfluidic system for the automated fabrication of cellular multilayers with enhanced extracellular matrix coatings.
  • To reduce the labor and mechanical stress associated with conventional multilayer fabrication techniques.

Main Methods:

  • A microfluidic system integrating channels, pumps, and valves was engineered.
  • The system was utilized to create nanometer-sized, layer-by-layer extracellular matrix films on C2C12 myoblast cell monolayers.
  • Cellular multilayers were fabricated within custom microfluidic channels and on standard culture dishes.

Main Results:

  • The microfluidic system successfully fabricated cellular multilayers with layer-by-layer extracellular matrix films.
  • Fabrication using the microfluidic system resulted in thicker multilayers compared to centrifugation methods.
  • The microfluidic approach significantly reduced labor input and minimized mechanical forces exerted on the cells.

Conclusions:

  • The developed microfluidic system offers a less laborious and gentler alternative for cellular multilayer fabrication.
  • This technology holds significant potential for applications in tissue modeling, pharmaceutical screening, and quantitative studies of cellular responses to stimuli.