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Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications
Raphael P H Meier1, Elisa Montanari2, Philippe Morel2
1Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland. Raphael.Meier@hcuge.ch.
Abstract:
Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels.
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