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Updated: Mar 12, 2026

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Using Coculture to Detect Chemically Mediated Interspecies Interactions
Published on: October 31, 2013
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A chemical reporter strategy for detecting and identifying O-mycoloylated proteins in Corynebacterium
Herbert W Kavunja1, Brent F Piligian1, Taylor J Fiolek1
1Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Abstract:
Protein O-mycoloylation is a unique post-translational lipidation that was recently discovered in Corynebacterium. We describe an alkyne-modified trehalose monomycolate chemical reporter that can metabolically tag O-mycoloylated proteins in C. glutamicum, enabling their detection and identification through click chemistry.
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