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Updated: Jul 22, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
[Ligase joining of oligodeoxynucleotides on a oligodeoxynucleotide analog matrix containing P-S-C(5')-internucleotide
Abstract:
Joining of oligodeoxynucleotides by T4 polynucleotide ligase on an oligodeoxynucleotide template analogue having P--S--C(5') internucleotide bonds was investigated. The enzyme did ligate the oligodeoxynucleotides but with an extremely low rate. The major product of the enzymatic reaction proved to be the adenylylated intermediate of the 5'-phosphorylated oligodeoxynucleotide, the product of the second stage of the overall ligation reaction. It is noteworthy that this intermediate is not accumulated in ligase reactions under standard conditions when DNA templates rather than template analogues were used.
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