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Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos
Canan Doganli1, Melissa Sandoval1, Sean Thomas2,3
1Cardiovascular Research Institute, University of California, San Francisco, CA, 94158, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2016
Abstract:
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is a useful method to map genome-wide chromatin accessibility and nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebrate embryos.
