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Updated: Aug 19, 2026

Electrophysiological Measurements and Analysis of Nociception in Human Infants
Published on: December 20, 2011
A multidimensional study of infants' behaviors associated with assumed painful stimuli: Phase II
Insights
Infants show physiologic pain responses, including crying and facial movements, when receiving vaccinations. Healthcare providers should use comprehensive assessments to evaluate infant pain accurately.
Area of Science:
- Neonatal research
- Pediatric pain assessment
- Infant behavioral responses
Background:
- Infant pain perception is a critical area of clinical and research interest.
- Understanding objective indicators of pain in non-verbal infants is essential for effective care.
Purpose of the Study:
- To identify and document observable physiologic and behavioral responses in infants experiencing pain.
- To provide evidence supporting the need for refined pain assessment tools in neonates.
Main Methods:
- Infants were audiovideotaped during their initial diphtheria-pertussis-tetanus (DPT) vaccination.
- Behavioral responses, including vocalizations and facial movements, were analyzed to identify pain indicators.
Main Results:
- Crying and specific facial movements were consistently observed during the DPT injection.
- These documented behaviors serve as indicators of infant pain perception.
Conclusions:
- Physiologic changes confirm that infants perceive pain.
- Nurses should employ multidimensional assessment strategies for infants suspected of experiencing pain.
Abstract:
Researchers have found that physiologic changes occur that indicate infants do perceive pain. In the study, infants were audiovideotaped while receiving the first diphtheria-pertussis-tetanus (DPT) injection to document their responses to an assumed painful stimulus. The vocalization of crying and some facial movements were identified as behaviors associated with receiving the DTP injection. These findings support the need for nurses to use a multidimensional assessment when caring for infants believed to be having pain.
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