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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Mar 12, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Microarray Technologies in Fungal Diagnostics.

Steffen Rupp^1,2

¹Fraunhofer Institut für Grenzflächen- und Bioverfahrenstechnik, Nobelstr. 12, Stuttgart, 70569, Germany. Steffen.Rupp@igb.fraunhofer.de.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2016

Summary

This summary is machine-generated.

Microarray technology enables rapid, multiparametric detection of biological compounds for research and diagnostics. These nucleic acid-based arrays offer advanced tools for infectious disease diagnostics, potentially replacing traditional methods.

Keywords:

DNA microarray Molecular diagnostics SNP analyses

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Last Updated: Mar 12, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Identification of Mycobacterium Species by DNA Microarray Chip Method

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

Area of Science:

Biotechnology
Molecular Diagnostics
Genomics

Background:

Microarray technology has evolved from nucleic acid detection to analyzing diverse biological compounds.
Advancements include closed-tube systems and lab-on-a-chip integration for enhanced automation and reduced contamination.
Microarrays are increasingly utilized in diagnostics, particularly for infectious diseases.

Purpose of the Study:

To review microarray technologies as research tools and diagnostic applications.
To highlight the role of nucleic acid-based arrays in modern diagnostics.
To discuss the potential of microarrays to supersede conventional diagnostic methods.

Main Methods:

Review of microarray technology principles and applications.
Focus on nucleic acid-based array systems.
Analysis of advancements in automation and detection capabilities.

Main Results:

Microarrays facilitate multiparametric detection of DNA, RNA, proteins, and other biomolecules.
Integration with lab-on-a-chip devices enhances automation and minimizes contamination risks.
Emerging biomarkers like noncoding RNAs and miRNAs expand diagnostic potential.

Conclusions:

Microarray technology is a versatile tool for both research and diagnostics.
Nucleic acid-based microarrays show promise in replacing traditional clinical microbiology assays.
The field is poised for further innovation with novel biomarkers and integrated systems.