Triterpenoids and lignans from the fruit of Schisandra sphenanthera

Three new (1-3) and seven known (4-10) triterpenoids, together with one new (11) and fifteen known (12-26) dibenzocyclooctadiene lignans, were isolated from the fruit of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by extensive analysis of spectroscopic methods, including HRESIMS, 1D and 2D NMR spectra and CD experiments. All of the isolated triterpenoids (1-10) were evaluated for their in vitro cytotoxicities against HepG2 human hepatocellular carcinoma cell line, and compounds 1, 3-7 and 10 exhibited moderate antiproliferative effects against HepG2 cell line with IC₅₀ ranging from 18.12 to 49.52μM. The lignans (11-26) were tested for their neuroprotective activities against CoCl₂, H₂O₂ and Aβ_25-35-induced SH-SY5Y cell injuries and showed considerable neuroprotective effects of different degrees. And at the low concentration of 3.2nM, compounds 14, 17-19, 23 in CoCl₂-induced, compounds 11, 13-15, 17, 19-20, 22-24 in H₂O₂-induced, and compounds 12-14, 19, 25-26 in Aβ_25-35-induced SH-SY5Y cell injury models, showed statistically significant neuroprotective activities compared with the negative control group, respectively.