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Related Experiment Video

Updated: Mar 12, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
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Methyl-binding DNA capture Sequencing for Patient Tissues

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Methyl-binding DNA capture Sequencing for Patient Tissues.

Rohit R Jadhav1, Yao V Wang1, Ya-Ting Hsu1

  • 1Department of Molecular Medicine, University of Texas Health Science Center at San Antonio.

Journal of Visualized Experiments : Jove
|November 15, 2016
PubMed
Summary

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DNA methylation is crucial for development and its dysregulation is linked to cancer. Methyl-Binding DNA Capture sequencing (MBDCap-seq) offers a method to quantify DNA methylation in large patient cohorts for cancer research.

Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • DNA methylation is a key epigenetic mechanism regulating gene expression essential for development and tissue differentiation.
  • Aberrant DNA methylation patterns are frequently observed in various diseases, particularly cancer.
  • Understanding methylation dynamics is critical for disease diagnostics and therapeutic strategies.

Purpose of the Study:

  • To introduce Methyl-Binding DNA Capture sequencing (MBDCap-seq) as a technique for quantifying DNA methylation.
  • To detail the protocol and bioinformatics pipeline for MBDCap-seq.
  • To highlight the application of MBDCap-seq in large-scale cancer methylome projects.

Main Methods:

  • Methyl-Binding DNA Capture sequencing (MBDCap-seq) utilizing an affinity enrichment approach.

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  • Development of a comprehensive bioinformatics pipeline for optimal methylation quantification.
  • Application of MBDCap-seq for analyzing DNA methylation across diverse cancer types.

    • Main Results:

    • MBDCap-seq enables precise quantification of DNA methylation in normal and disease tissues.
    • The described protocol and pipeline facilitate large-scale patient cohort analysis.
    • The technique has been successfully applied in the 1,000 methylome project for cancer research.

    Conclusions:

    • MBDCap-seq is a valuable tool for large-scale DNA methylation analysis in human tissues.
    • This technique aids in identifying methylation dysregulation associated with diseases like cancer.
    • MBDCap-seq contributes to advancing cancer epigenomics research.